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In Vitro Services
Expert study designs and data interpretation from the In Vitro services group at Agilux allow you to characterize your drug candidate's ADME properties and its potential for drug interactions with our in vitro ADME assays.
Agilux Laboratories has focused on building systems and procedures to meet the needs and timelines of clients working in the discovery environment. Our goal is to deliver "Better Data Faster”. We offer a range of Research Grade Assays (RGAs) in our in-vitro assay services. Each of our in vitro assays can be offered as an early screening single determination (RGA 1) or a more definitive study to determine PK/ ADME characteristics (RGA 2 & RGA 3). Each of the assays has positive controls that are monitored for inter-day variability as well as acting as controls for that specific analysis. While Agilux has standard procedures for each of these assays, our team of experienced Study Directors can also assist in designing the best strategy and protocols customized to suit any drug discovery needs.
For more information on our In Vitro services, please see the details below and contact us at info@agiluxlabs.com.
View the In Vitro Product Specification.
- Human recombinant enzymes
- Major CYP450 isozymes: 1A2, 2C8, 2C9, 2C19, 2D6, 3A4
- IC50 determinations
- Time-dependent inhibition (IC50 shift)
- Human liver microsomes
- CYP450-selective inhibitors
- Metabolite monitoring
- Multiple CYP450 isozymes
- Cryopreserved hepatocytes
- Plasma, serum, whole blood, microsomes or purified proteins (albumin, AGP)
- Human, monkey, rodent, dog, swine and other species upon request
- Initial screening assessments or definitive binding determinations
- Routine screening support or custom studies
- Incubate test article +/- melanin
- Separate free compound from melanin-bound by centrifugation
- Synthetic melanin
- Also available from sepia (cuttlefish)
- Initial screening assessments or definitive binding determinations
- Liver microsomes, S9, plasma, whole blood, aqueous buffers
- Human, monkey, rodent, dog, swine and other species upon request
- Initial screening assessments or definitive determinations (T1/2, CLint)
- Metabolite monitoring
- Human, cynomolgus monkey, rat, mouse, dog, swine, and other species upon request
- Fresh primary hepatocytes available upon request
- Initial screening assessments or definitive determinations (T1/2, CLint)
- Option to simultaneously monitor for multiple metabolite masses (selected by Sponsor or proposed from compound structure)
- Human, cynomolgus monkey, rat, mouse, dog, swine, and other species upon request
- Fresh primary hepatocytes available upon request
- Initial screening assessments or definitive determinations (T1/2, CLint)
- Option to simultaneously monitor for multiple metabolite masses (selected by Sponsor or proposed from compound structure)
- % Remaining in Ex Vivo matrices (plasma, serum, CSF), aqueous buffers
- Fresh whole blood
- Multiple species
- Caco-2 is a human epithelial colorectal adenocarcinoma cell line
- Cell monolayer obtained commercially
- Incubate test article on apical side of cell monolayer for 1-2 hours
- Measure analyte levels on apical and basolateral sides of cell monolayer using LC-MS/MS bioanalysis
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CYP450 Inhibition Assay Services
